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Wavelength Range - 200 to 900 nm
Polychromator
Light Source – Xenon flash lamp
Measure Time for full scan range- 3.5-6.0 seconds
Wavelength Accuracy - ± 0.75 nm (N50: 1.5 nm)
Working Principle
Ultraviolet-visible (UV-Vis) spectrophotometer is used to quantify and qualify samples by the means of UV and visible light (mainly 200 to 900 nm). In comparison to the first commercially available UV/Vis spectrophotometer, which has been a monochromatic scanner, the NanoPhotometer represents a new class of UV-Vis spectrophotometer instruments with a polychromatic rather than a monochromatic optical setup.
The full spectrum of light produced by a Xenon Flash Lamp is sent through the sample and then separated after it is passed using a polychromator. To record the signals, modern UV-Vis spectrophotometers are utilizing a CCD or CMOS detector (linear camera), which allows to record high-resolution spectra over the entire wavelength range within a few seconds even.
Key Features
(i) For Nanovolume
Detection Range dsDNA 1 – 16,500 ng/µl
Detection Range BSA 0.03 – 478 mg/ml
Minimum Sample Size 0.3 – 2 µl
Photometric Range (10 mm equivalent) 0.02 – 330 A
Path Length 0.67 and 0.07 mm
Dilution Factor 15 and 140
Vortex 2,800 rpm; tube size up to 2.0 ml
(ii) For Cuvette
Detection Range dsDNA 0.1 – 130 ng/µl
Detection Range BSA 0.003 – 3.7 mg/ml
Photometric Range 0 – 2.6 A
Center Height (Z-Height) 8.5 mm
Cell Types Outside dimension 12.5 x 12.5 mm
Heating 37°C ± 0.5°C
Optical Specifications
Wavelength Scan Range 0.1 – 130 ng/µl
Measure Time For Full Scan Range 0.003 – 3.7 mg/ml
Wavelength Reproducibility 0 – 2.6 A
Wavelength Accuracy 8.5 mm
Bandwidth Outside dimension 12.5 x 12.5 mm
Optical Arrangement 37°C ± 0.5°C
Lamp Outside dimension 12.5 x 12.5 mm
Contact Us
CRNTS/SAIF IIT Bombay
Contact: 022-21593067
Email Id : manaliv@iitb.ac.in
Nano UV-Visible Photometer system charges excluding 18% GST